Transcriptional Regulation of icaADBC by both IcaR and TcaR in Staphylococcus epidermidis
نویسندگان
چکیده
منابع مشابه
icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis.
Biofilm formation in Staphylococcus epidermidis is dependent upon the ica operon-encoded polysaccharide intercellular adhesin, which is subject to phase-variable and environmental regulation. The icaR gene, located adjacent to the ica operon, appears to be a member of the tetR family of transcriptional regulators. In the reference strain RP62A, reversible inactivation of the ica operon by IS256...
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The proteins encoded by the intercellular adhesin genes (icaADBC) synthesize the polysaccharide poly-N-acetylglucosamine (PNAG), which contributes to the formation of a biofilm by Staphylococcus aureus. In addition to the proteins that synthesize PNAG, the ica locus also encodes the TetR family transcriptional regulator IcaR (23). The icaR gene is transcribed divergently from icaADBC (10) and i...
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Biofilm formation in Staphylococcus epidermidis is mediated by icaADBC-dependent and -independent pathways. Subinhibitory concentrations of erythromycin, azithromycin, and clarithromycin enhanced, in a dose-dependent manner, the level of biofilm formation by 20% (21/105 isolates) by macrolide-resistant ica-positive and -negative isolates tested in vitro. The presence of ica, however, apparently...
متن کاملStructural study of TcaR and its complexes with multiple antibiotics from Staphylococcus epidermidis.
TcaR and IcaR are a weak and a strong negative regulator of transcription of the ica locus, respectively, and their presence prevents the poly-N-acetylglucosamine production and biofilm formation in Staphylococcus epidermidis. Although TcaR was shown to interact with the ica promoter, the precise binding region and the mechanism of interaction remained unclear. Here we present the 3D structure ...
متن کاملThe teicoplanin-associated locus regulator (TcaR) and the intercellular adhesin locus regulator (IcaR) are transcriptional inhibitors of the ica locus in Staphylococcus aureus.
Infections involving Staphylococcus aureus are often more severe and difficult to treat when the organism assumes a biofilm mode of growth. The polysaccharide poly-N-acetylglucosamine (PNAG), also known as polysaccharide intercellular adhesin, is synthesized by the products of the intercellular adhesin (ica) locus and plays a key role in biofilm formation. Numerous conditions and exogenous fact...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2019
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00524-18